The Effect of Microalgae Supplement with Herbal Powder and Conjugated Linoleic Acid (CLA) on Production Performance, Blood Parameters and Ovarian Activity in Laying Hens

This study aimed to investigate the effects of dietary supplementation with microalgae, herbal powder, and conjugated linoleic acid (CLA) on production performance, blood parameters, and ovarian activity in Loh-man laying hens. Sixty 43-week-old hens (1.6±0.2 kg) were randomly assigned to four dietary groups for 42 days: a control group (no additives) and three experimental groups receiving 0.1%, 0.2%, or 0.3% of a supplement blend containing Spirulina, Chlorella, CLA, and herbal powders (dill and peppermint). Parame-ters including production performance, egg weight, serum lipids, ovarian follicle activity, and liver fat were evaluated. Results indicated that the highest supplementation level (0.3%) significantly improved produc-tion performance and egg weight compared to the control and other groups (P<0.05). On day 42, serum lipid analysis showed a significant reduction in cholesterol levels in the 0.3% group (P<0.05). Ovarian fol-licle activity increased with higher supplementation levels, showing a statistically significant enhancement over the control (P<0.05). Liver evaluation revealed a notable decrease in total liver fat in the supplemented groups, particularly at the 0.3% level (P<0.05). In conclusion, dietary inclusion of 0.3% microalgae, CLA, and herbal supplements enhanced productive performance, improved egg quality, optimized serum choles-terol levels, supported ovarian activity, and contributed to better liver health in Lohman laying hens.