Innovative Gel Formulation with 70% Ethanol Extract of Pandanus amaryllifolius Leaf for Accelerated Healing of Second-Degree Burn in Rats

Burns, particularly second-degree burns, are common injuries faced by many people. Pandan Wangi (Pandanus amaryllifolius Roxb), a plant known for its medicinal properties, has shown potential in treating burns. This study aimed to evaluate the potential of 70% ethanol leaf extract of Pandanus amaryllifolius gel formulation as a natural ingredient with strong wound-healing properties. Pandanus amaryllifolius leaf was extracted by maceration in 70% ethanol. The extract was subjected to qualitative phytochemical screening following standard procedures. Three gel formulations containing Pandanus amaryllifolius leaf extract at concentrations of 30% (F1), 35% (F2), and 40% (F3) were prepared using Viscolam MAC 10 as the gel base. The physicochemical properties (organoleptic, viscosity, pH, spreadability, and adhesion),  irritation, and stability of the gel formulations were evaluated. The burn wound-healing activity of the gel formulations was evaluated in rats. The antibacterial activity of the gel formulations was evaluated against Staphylococcus aureus and Pseudomonas aeruginosa using the agar well diffusion method. Phytochemical screening of Pandanus amaryllifolius leaf extract revealed the presence of flavonoids, alkaloids, saponins, and tannins. The gel formulations demonstrated favorable physicochemical properties. Among the formulations, F3 containing 40% extract, showed the most rapid burn wound healing, reducing the recovery period by 9 days. F3 also demonstrated significant antibacterial activity against both Staphylococcus aureus and Pseudomonas aeruginosa with inhibition zone diameter of 6.0 and 3.8 mm, respectively. This study supports the potential of Pandanus amaryllifolius as a natural ingredient with strong wound-healing properties, further contributing to the development of herbal-based formulations for burn care.