Comparative evaluation of two MALDI-TOF MS systems for microbial identification: accuracy and workflow efficiency in a clinical microbiology laboratory

This study provides a real-world comparison of two leading matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems, VITEK MS PRIME (VMP) and MALDI Biotyper Sirius (BS), based on 927 isolates across 219 species, including rare and challenging organisms. By simulating routine lab workflows working with several workstations, it offers practical insights into hands-on time and time to results. Unlike previous studies, it includes a broader sample set (including yeasts), evaluates short positive blood culture incubation conditions, and accounts for operator variability. These strengths make it a valuable resource for laboratories seeking evidence-based guidance on MALDI-TOF implementation and optimization.