Disposable Electrochemical Biosensor for Determination of Dibutyl Phthalate Migrated from Food Packaging Materials

In this work, an electrochemical aptasensor for sensitive and selective determination of dibutyl phthalate (DBP) was fabricated by modifying the working electrode with poly(glycidyl methacrylate) (PolyGma) and DBP aptamer. The DBP aptamer was employed as a biorecognition probe, and the PolyGma polymer was a suitable matrix for DBP aptamer immobilization due to its high number of epoxy groups. The biosensor fabrication strategy was based on the covalent binding of DBP‐specific aptamer on the working electrode surface. Several electrochemical methods were utilized to characterize various steps of aptasensor fabrication. The introduction of DBP on the biosensor surface led to specific interaction and changed the aptasensor response. The DBP level was quantified using electrochemical impedance spectroscopy. The proposed assay was permitted to detect DBP in the linear range from 1 to 200 pg/mL with a very low limit of detection (0.295 pg/mL). The suggested aptasensor illustrated high selectivity in the presence of other endocrine disruptors at a fourfold concentration of DBP, and furthermore, it showed long storage stability. In addition, this aptasensor might be effectively regenerated and reused. This aptasensor was also used to quantify DBP levels in DBP‐migrated water samples from food packaging materials, with good recoveries ranging from 95.73% to 104.13%. In comparison with the gas chromatography approach, the manufactured aptasensor was ultimately successful in measuring DBP.