Biphasic extraction metabolites in algal matrix or periphyton v2

The purpose of this instruction is to specify the different steps of extraction of metabolites and lipids on an algal matrix or periphyton previously freeze-dried and stored in a freezer at . Principle: Use of a MeOH/MTBE/H2O mixture for the simultaneous extraction of primary, secondary and lipid metabolites. Extraction is done by combining liquid/solid and liquid/liquid extraction that is carried out twice to optimize yields.