Porous tube Sorptive extraction: A robust sol–gel sorbent encapsulated device for monitoring bisphenols in human urine

Α new microextraction platform Porous Tube Sorptive Extraction (PTSE) is reported for the first time for the selective isolation of bisphenols from human urine prior to HPLC–DAD analysis. The novel device consists of a micro-porous tubular substrate uniformly coated with a sol–gel Polyethylene Glycol 20 K (sol-gel PEG 20 K) sorbent phase, providing a high surface area, hydrophilic–lipophilic network that promotes efficient partitioning of target analytes while minimizing matrix effects. Key operational variables were optimized using a one-factor-at-a-time (OFAT) strategy, and the PTSE–HPLC–DAD workflow was validated in human urine. The method delivered high recoveries (71.0–100.9%) , good linearity across the working range, and low quantitation and detection limits of 0.05 ng/μL and 0.015 ng/μL, respectively ( ≈50 ng/μL and 15 ng/ μ L ). The PTSE device operated with minimal organic solvent and supported multiple extraction-desorption cycles without appreciable loss of performance, underscoring its robustness and cost-efficiency. Method greenness was quantitatively confirmed using Modified Green Analytical Procedure Index ( MoGAPI) and Blue Applicability Grade Index ( BAGI) metrics, evidencing reduced solvent consumption and waste generation relative to conventional approaches. Overall, PTSE combines selectivity, sensitivity, and sustainability in a compact format compatible with routine HPLC–DAD analysis, positioning it as a practical tool for bisphenol biomonitoring and a promising template for future sol–gel tube-based microextraction devices. • • A novel Porous Tube Sorptive Extraction (PTSE) device was developed using sol–gel PEG 20 K. • • PTSE enables selective and efficient extraction of bisphenols from human urine. • • Method delivers high recoveries (71.0–100.9%), excellent linearity (R 2 > 0.998), and low LOD/LOQ of 0.015/0.05 ng/uL. • • MoGAPI and BAGI scores confirm excellent greenness and practical applicability. • • PTSE–HPLC–DAD offers a robust, low-cost extraction method for bisphenol biomonitoring.