Biological Stability and Microbial Recovery Responses in Vermicomposting of Chemically Intensive Tomato Residues: Defining Management Limits

The intensive cultivation of greenhouse tomatoes generates massive quantities of vegetative residues often laden with potentially complex pesticide contaminants, posing a dual challenge of waste management and environmental toxicity. This study investigated the biological feasibility and system tolerance of valorizing these hazardous residues through vermicomposting with <i>Eisenia fetida</i>, using mixtures of cattle manure and tomato residues (TR) at varying ratios (0-60%) over a 45-day incubation period. The process was monitored through physicochemical parameters (pH, EC, C/N ratio) and sensitive biological indicators (Basal Respiration and Microbial Biomass Carbon). While TR inclusion rates exceeding 30% induced acute inhibitory effects (100% mortality within 5 days) due to acute toxicity, mixtures containing up to 30% were successfully processed. The biological monitoring revealed a distinct "biphasic response": an initial "metabolic lag phase" (days 0-15) driven by chemical stress, followed by a robust "biological recovery" where microbial activity surged significantly after day 30. Correlation analyses confirmed that this recovery was mechanically linked to the acidification of the substrate, as indicated by strong negative correlations between pH and biological activity (<i>r_s</i> = -0.70). Ultimately, vermicomposting significantly reduced Electrical Conductivity (EC) and lowered the C/N ratio below 15 in all viable treatments, confirming the stabilization of waste into an agronomically mature product. The results demonstrate that the earthworm gut functions as an effective bioreactor, facilitating biological stabilization and the mitigation of toxicity in pesticide-laden biomass. This study concludes that vermicomposting is a robust strategy for converting toxic agro-wastes into a stabilized organic amendment, provided that the residue load is managed within the identified physiological tolerance threshold of 30%.