Adaptive responses of genome-reduced Bacillus subtilis during enzyme secretion in a breathing vessel culture
While the overall AmyQ yields per liter were similar in both fermentation systems, the total enzyme yields in the breathing fermenter were significantly higher due to the 15-fold increase in culture volume. Our findings imply that breathing fermentation vessels are suitable for B. subtilis enzyme production and, upon further scale-up, they may represent sustainable and cost-effective alternatives to traditional fermentation systems for microbial cell factories.
Authors
- Yuzheng Wu (ORCID: https://orcid.org/0000-0002-7817-329X)
- Ayşegül Öktem (ORCID: https://orcid.org/0000-0001-7828-999X)
- Francesca Grilli (ORCID: https://orcid.org/0000-0001-9579-5704)
- Jan Maarten van Dijl
- Sandra Maaß
- Shu Ishikawa
- Ryosuke Sato
- Ken-ichi Yoshida
- Masato Yotsuya
- Kyosuke Yokoyama
Institutions
- University Medical Center Groningen (NL)
- University of Groningen (NL)
- Kajima Corporation (Japan) (JP)
- Universität Greifswald (DE)
- Kobe University (JP)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2026-01-29
- DOI
- https://doi.org/10.1186/s12934-026-02942-x
- Primary Topic
- Bacterial Genetics and Biotechnology
- Type
- article
- Field-Weighted Citation Impact
- 0.00