Molecular determinants of antibody-mediated priming to enhance detection of ctDNA
Low concentration of ctDNA limits the sensitivity of liquid biopsies in many applications. This study shows how engineered antibodies and other dsDNA-binding molecules can inhibit clearance of ctDNA from the bloodstream and increase concentration of ctDNA in a blood draw, and identifies the key features that enable this activity. Modulation of cfDNA in the bloodstream using these agents can increase recovery of ctDNA and improve the performance of liquid biopsies for cancer detection.
Authors
- Viktor A. Adalsteinsson (ORCID: https://orcid.org/0000-0003-4555-2485)
- Timothy Blewett
- Daniel M. Kim (ORCID: https://orcid.org/0000-0001-6033-4975)
- Kasturi Chakraborty (ORCID: https://orcid.org/0000-0002-0635-9028)
- Shervin Tabrizi (ORCID: https://orcid.org/0000-0003-2780-8432)
- Savan K. Patel (ORCID: https://orcid.org/0000-0002-8909-9144)
- Ruolin Liu (ORCID: https://orcid.org/0000-0001-9059-6335)
- Carmen Martin-Alonso (ORCID: https://orcid.org/0000-0001-6581-7909)
- Justin Rhoades (ORCID: https://orcid.org/0000-0002-0630-908X)
- Andjela Crnjac (ORCID: https://orcid.org/0009-0005-9651-4288)
- Sahil Patel (ORCID: https://orcid.org/0000-0002-8975-900X)
- Claire Sullivan
- J. Christopher Love
- Zhenyi An
- Sangeeta N. Bhatia
- Lily Gao
- Kan Xiong
Institutions
- Broad Institute (US)
- Boston University (US)
- University of Southern California (US)
- Biogen (United States) (US)
- Howard Hughes Medical Institute (US)
- Harvard University (US)
- Harvard–MIT Division of Health Sciences and Technology (US)
- Exact Sciences (United States) (US)
- Mass General Brigham (US)
- Massachusetts Institute of Technology (US)
- University of Pennsylvania (US)
Publication Details
- Journal
- bioRxiv (Cold Spring Harbor Laboratory)
- Published
- 2026-01-29
- DOI
- https://doi.org/10.64898/2026.01.27.701975
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Prostate Cancer Foundation
- Massachusetts General Hospital
- Dana-Farber/Harvard Cancer Center
- Gerstner Family Foundation
- Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
- National Institutes of Health
- National Cancer Institute