A surrogate‐selection‐enhanced TnpBmax platform enables efficient genome editing in rice and tomato
TnpB, a compact RNA-guided nuclease ancestral to Cas12, is optimized for plant genome editing. A 99-nt enhanced RNA and T5 exonuclease fusion generate TnpBe5, boosting rice editing 2.5-fold. Coupling with a single-strands annealing (SSA)-responsive HYYG surrogate (TnpBmax) enriches edited cells, achieving up to 81.5% efficiency and high homozygosity across rice and tomato.
Authors
- Jiyong Xie
- E. F. Li
- Zai Zheng (ORCID: https://orcid.org/0000-0001-5773-9402)
- Yifu Tian (ORCID: https://orcid.org/0000-0002-8167-5151)
- Mengdi Wang (ORCID: https://orcid.org/0000-0002-2101-9507)
- Xinbo Li
Institutions
- South China Agricultural University (CN)
- Chinese Academy of Tropical Agricultural Sciences (CN)
- Southern University of Science and Technology (CN)
- Chinese Academy of Agricultural Sciences (CN)
- Ministry of Agriculture and Rural Affairs (CN)
- Institute of Crop Sciences (CN)
Publication Details
- Journal
- Journal of Integrative Plant Biology
- Published
- 2026-01-28
- DOI
- https://doi.org/10.1111/jipb.70159
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- China Postdoctoral Science Foundation