Development of a standardized murine model for investigating cholesterol crystallization and deposition in the gallbladder wall: effects of dietary cholesterol and pharmacological modulation

Impaired mucosal cholesterol reverse transport, particularly under combined inhibition of both mucin expression and RCT, was associated with the most pronounced submucosal lipid deposition and the highest incidence of gallstone formation. To our knowledge, this study is the first to establish an animal model of GCPs integrating mucoprotein and RCT regulation, providing a platform for mechanistic studies and potential therapeutic exploration.