Uncovering the genomic complexity of <i>PAX5</i> intragenic tandem multiplication via long-read and short-read sequencing
By integrating short-read whole-genome sequencing and RNA sequencing (RNA-seq) data with long-read RNA-seq, we dissect the complex genomic architecture of PAX5 intragenic tandem multiplication, revealing that these complex rearrangements result in in-frame transcripts that likely encode proteins with altered domains.
Authors
- Patrick A. Brown
- Lingyun Ji (ORCID: https://orcid.org/0000-0003-2500-1993)
- W L Carroll
- Heather L. Mulder (ORCID: https://orcid.org/0000-0003-2024-9498)
- John M. Easton (ORCID: https://orcid.org/0000-0001-8745-6753)
- Ti-Cheng Chang
- Soheil Meshinchi (ORCID: https://orcid.org/0000-0002-6276-4423)
- Y. Liu
- Tyler Jones
- Wenan CHEN
- Huanbin Zhao
- Melanie R. Loyd
- Pandurang Kolekar (ORCID: https://orcid.org/0000-0003-0044-0076)
- Andrea McBride
- Christian Deardorff
- D. W. Bennett
- Hanxia Li
- Rhonda E. Ries
- Xiaotu Ma
- Jun J Yang
- Samuel W Brady
- Bensheng Ju
- Charles G. Mullighan
- Mignon L. Loh
- Emily Michelle Plyler
- Monta Briviba
- Jinghui Zhang
- Li Fan
- Gang Wu
- Alexis Eckert
- Geoff Neale
- Yuan Feng
- Li Dong
Institutions
- University of Southern California (US)
- St. Jude Children's Research Hospital (US)
- Nationwide Children's Hospital (US)
- Latvian Biomedical Research and Study Centre (LV)
- NYU Langone Health (US)
- Fred Hutch Cancer Center (US)
- Seattle Children's Hospital (US)
- Center for Cancer and Blood Disorders (US)
- Haemonetics (United States) (US)
Publication Details
- Journal
- Blood
- Published
- 2026-01-26
- DOI
- https://doi.org/10.1182/blood.2025031289
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Leukemia and Lymphoma Society
- American Lebanese Syrian Associated Charities
- National Institutes of Health
- National Cancer Institute