Wnt10a exhibit spatiotemporal singularity in the temporal changes of angiogenesis in regenerated pulp-like tissue

During angiogenesis in pulp regeneration, when SHED differentiate into blood vessels, Canonical Wnt signaling and VEGF-A are activated to form microvessels, and Tie-2 expression is enhanced to increase vessel circumference. Furthermore, Wnt10a was found to be activated in its early stages and decreased in its mature stages, with spatio-temporal specificity. Because its expression is very low, Wnt10a could be a biomarker to monitor the regenerative status of pulp regeneration.