Isolation of Chicken Intestinal Glial Cells and Their Transcriptomic Response to LPS

Current research on glial cells has primarily focused on central nervous system glial cells (CNS glia), with relatively fewer studies on EGCs. Given the critical role of EGCs in maintaining intestinal homeostasis and neural function, this study aimed to investigate their immunomodulatory effects under inflammatory conditions. Primary EGCs were isolated and an inflammatory model was established by treatment with lipopolysaccharide (LPS). Following LPS induction, cellular samples were collected for transcriptomic analysis to identify differentially expressed genes. The analysis revealed that 88 genes were significantly altered, with 60 upregulated and 28 downregulated. Through Gene Ontology (GO) classification, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway mapping, and protein-protein interaction (PPI) network analysis, several key regulatory genes were identified: chemokine-related genes (<i>IL8L2</i>, <i>IL8L1</i>, <i>CCL4</i>, <i>CCL5</i>, and <i>CX3CL1</i>); negative feedback regulation-related genes (<i>TNFAIP3</i> and <i>ZC3H12A</i>); homeostasis-maintaining genes (<i>C1QB</i> and <i>LY86</i>); and arachidonic acid metabolism-related genes (<i>PTGS2</i> and <i>GGT2</i>). Under LPS stimulation without impairing EGC viability, EGCs may recruit immune cells by regulating the aforementioned genes. Additionally, arachidonic acid and its metabolites likely play important regulatory roles in EGC-mediated immunomodulation. These findings provide new theoretical insights and potential targets for further elucidating the pathogenesis of intestinal inflammation and developing targeted therapies.