Establishing hospital-specific background microbial libraries to reduce false positives in mNGS diagnosis of periprosthetic joint infection

The composition and abundance of residual bacterial DNA vary markedly among institutions, underscoring the necessity of establishing hospital-specific BMLs. Incorporating such libraries into clinical mNGS interpretation can effectively reduce false positives and enhance the diagnostic accuracy of PJI. arthroplasty, bacterial culture, next-generation sequencing, joint replacement, periprosthetic joint infection, background microbial libraries.

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Publication Details

Journal
Frontiers in Cellular and Infection Microbiology
Published
2026-01-26
DOI
https://doi.org/10.3389/fcimb.2025.1668697
Primary Topic
Orthopedic Infections and Treatments
Type
article
Field-Weighted Citation Impact
0.00

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article

Establishing hospital-specific background microbial libraries to reduce false positives in mNGS diagnosis of periprosthetic joint infection

Chengtan Wang, Duliang Xu, Zhenfeng Yuan, Wenbin Xu et al.
Frontiers in Cellular and Infection Microbiology
Orthopedic Infections and Treatments
article

Establishing hospital-specific background microbial libraries to reduce false positives in mNGS diagnosis of periprosthetic joint infection

Chengtan Wang, Duliang Xu, Zhenfeng Yuan, Wenbin Xu, Yinguang Cao, Han Yin, Feng Pang, Wei Li, Dawei Wang, Zhenzhu Song
article en

Abstract

The composition and abundance of residual bacterial DNA vary markedly among institutions, underscoring the necessity of establishing hospital-specific BMLs. Incorporating such libraries into clinical mNGS interpretation can effectively reduce false positives and enhance the diagnostic accuracy of PJI. arthroplasty, bacterial culture, next-generation sequencing, joint replacement, periprosthetic joint infection, background microbial libraries.

Frontiers in Cellular and Infection MicrobiologyVol. 15
Chinese Academy of Medical Sciences & Peking Union Medical College (CN), Peking University (CN), National Health and Family Planning Commission (CN), Beijing Jishuitan Hospital (CN), Liaocheng People's Hospital (CN)
Wu Jieping Medical Foundation
Openalex Percentile: Top 77%
Orthopedic Infections and Treatments
DOIOpen Access PDFOpenAlex
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Establishing hospital-specific background microbial libraries to reduce false positives in mNGS diagnosis of periprosthetic joint infection — Chengtan Wang, Duliang Xu, et al. · Frontiers in Cellular and Infection Microbiology (2026) | TGRS Research Map | TGRS