Nucleocapsid protein captures DDX5 and RNMT facilitating viral RNA synthesis and viral protein translation for coronavirus replication
The synthesis of viral RNA and proteins is a crucial process in the life cycle of CoVs. Our observations indicate that DDX5 and SND1 facilitate the assembly of viral replication-transcription complexes and enhance viral RNA synthesis, with DDX5 binding to positive-sense RNA and SND1 binding to negative-sense RNA. Meanwhile, RNMT promotes viral protein translation by hijacking the host translation machinery and mediating the circularization of viral mRNA. These findings offer new insights into the mechanisms through which coronaviruses exploit both viral and host proteins to synthesize viral RNA and proteins.
Authors
- Tong Wu (ORCID: https://orcid.org/0009-0008-1435-1481)
- Wenzhen Qin (ORCID: https://orcid.org/0009-0005-7497-0362)
- Xinyu Yang (ORCID: https://orcid.org/0000-0001-5498-1877)
- Hai Yu (ORCID: https://orcid.org/0000-0001-8515-3134)
- C. Xinhong Wang
- Ying Liu
- Ai Gao (ORCID: https://orcid.org/0000-0002-4278-5040)
- D. Zheng (ORCID: https://orcid.org/0009-0002-7539-9717)
- Tongling Shan
- Ning Kong
- Guangzhi Tong
- Yahe Wang
- He Sun
- Hao Zheng
- Jiarui Wang
Institutions
- Shanghai Veterinary Research Institute (CN)
- Chinese Academy of Agricultural Sciences (CN)
- Yangzhou University (CN)
Publication Details
- Journal
- mBio
- Published
- 2026-01-26
- DOI
- https://doi.org/10.1128/mbio.02717-25
- Primary Topic
- Animal Virus Infections Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Huazhong Agricultural University