The Influence of Ketoprofen Use on the Activity of Superoxide Dismutase in Rat’s Liver

Abstract Ketoprofen (K) is a popular nonsteroidal antiinflammatory drug (NSAID) used for postoperative and acute pain. Its’ use can lead to side effects like bleeding from the gastrointestinal tract, hypertension, oedema, photodermatitis. It is metabolized in the liver and eliminated with urine. The aim of the study was to measure the effect of K on blood morphology, biochemical blood parameters and effect on oxidative stress in rat’s liver by measuring the activity of superoxide dismuthase (SOD). The experiment was accepted by the Local Ethical Committee (decision No 70/2021). A group of 12 female rats were used. They were randomly divided into 2 groups of 6: 1.controls which received saline by gavage for 5 days 2.study group receiving K at the dose of 8 mg/kg b.w. by gavage for 5 consecutive days. On day 6 all animals were decatpitated. Their blood and livers were obtained. Blood morphology, alanine transaminase (ALT) activity were measured. The neutrophil - lymphocytes ratio (NLR), platelet-lymphocytes ratio (PLR) and monocytes-lymphocytes ratio (MLR) were calculated. SOD activity was measured in the livers with an Elisa kit. There was no significant differences among the groups in blood morphology (except for eosinophil and neutrophil count, which were significantly elevated in the group exposed to K), ALT nor SOD activity. Significant differences were recorded in NLR, PLR and MLR, which were higher in the group exposed to K. NLR, PLR and MLR are new prognostic parameters used in monitoring inflammatory, autoimmune diseases and some neoplasms. Conclusions: 1. K does not produce oxidative stress in the liver and athe the therapeutic dose does not affect neither ALT nor SOD activity. 2. NLR, PLR and MLR are new interesting and cheap markers of inflammatory, autoimmune and neoplastic disorders.