The Effect of Ketoprofen Lysine Salt on Blood Morphology, Biochemical Blood Parameters and Superoxide Dismuthase Activity in Rat’s Kidney

Abstract Ketoprofen lysine salt (KLS) is a new nonsteroidal antiinflammatory drug (NSAID) of better solubility and better tolerability than older NSAIDs. It is metabolized in the liver and eliminated from the organism with urine. Like all NSAIDs KLS has possible side effects on the gastrointestinal tract, cardiovascular system and the kidney. The aim of the study was to measure the effect of KLS on blood morphology, biochemical blood parameters and superoxide dismuthase (SOD) activity in rat’s kidney. The project of the experiment was accepted by the Local Ethical Committee (70/2021). A total of 12 young female Wistar rats were used. They were randomly divided into 2 groups: controls receiving 0,9% NaCl by gavage and study group receiving KLS by gavage at the dose of 12.8 mg/kg b.w. for 5 consecutive days. On day 6 the animals were decapitated. Their blood and kidneys were obtained. Blood morphology and serum creatinine were measured. The neutrophil - lymphocytes ratio (NLR), platelet-lymphocytes ratio (PLR) and monocytes-lymphocytes ratio (MLR) were calculated. The SOD activity was measured in the kidneys with an Elisa kit. There was no significant differences among the groups in blood morphology (except for neutophil and eosinophil count, which were significantly elevated in the group exposed to KLS), creatinine concentration nor SOD activity. However there were significant differences in NLR it was much higher in the KLS group. NLR is considered to be a good prognostic parameter used in monitoring inflammatory and neoplastic diseases. Conclusions: 1 KLS does not significantly affect the activity of SOD in the kidneys suggesting the it does not produce much oxidative stress. 2. NLR is a new interesting and cheap marker of inflammatory processes.