Study the Most Predominant Bacteria in Pericoronitis Patients

Abstract Background: Pericoronitis is a term used to describe inflammation of the soft tissues surrounding the crown of a tooth that is erupting or has an incomplete eruption. The development of mixed oral microflora appears to be one of the causes of pericoronitis, a bacteria-induced infection of the soft tissues of the mouth that encircle the crown of impacted or partly erupted mandibular third molars. Objectives: The purpose of this research was to discover the main pathogenic microbes linked to pericoronitis by molecular methods. Materials and Methods: A total of 80 study samples (50 cases; 30 control), 34 men and 46 women, aged 16–36 years. During the acute stage of pericoronitis, samples are collected by isolation of the lower third molar and dry area by air syringe and then a sterile 3-paper point is inserted in pericoronal pockets. After that, the paper point is put in an Eppendorf tube and stored at deep freeze until it was used for real-time polymerase chain reaction (PCR) technique. Results: The most common bacteria detected in real-time-PCR are Streptococcus mutants , Prevotella intermedia , and Tanerella forthysia . Our results in pericoronitis show that the percentage of gram-positive bacteria S. mutans is 64%, gram-negative P. intermedia 58%, and T. forthysia 36%. Conclusion: Bacteria detection was found to be remarkably high compared to that of controls. Distribution of infection according to gender is (66%) women and (34%) men, and the number of patients with pericoronitis at a young age (16–26) is significantly higher than in the older age groups.