Genotyping of Chlamydia trachomatis by OMP1 Sequencing in Patients with Pelvic Inflammatory Disease

Abstract Background: Chlamydia trachomatis is one of the most sexually transmitted pathogens. Significant reproductive effects, such as infertility, can result from chlamydial infection, which emerges from the lower vaginal canal. This sexually transmitted micro-organism also contributes significantly to pelvic inflammatory disease (PID), which has been associated with a higher risk of infertility. Additionally, sequence typing was used to distinguish C. trachomatis strains and genotyping by using the widely used ompA gene polymorphism, a single-locus typing standard. This method, which is frequently employed for genotyping a variety of microorganisms, including the assessment of Chlamydia diversity, is based on the examination of polymorphism of housekeeping genes. Samples from symptomatic C. trachomatis -infected patients, were collected for this cross-sectional pilot study. Objectives: This work investigates the analyzed sequencing of C. trachomatis by using OMP1 polymerase chain reaction (PCR) sequencing during Chlamydia in women with PID. Materials and Methods: The study included 200 endocervical swab samples from patients with PID. Patients’ age ranged from 20 to 45 years. Chlamydia trachomatis was determined by convention PCR, whereas genotyping was done by OMP1 gene by PCR sequencing. Results: Twenty two (22/200) endocervical swabs were positive for C. trachomatis and only 20 were studied by sequencing of C. trachomatis OMP1 gene. The results also revealed that there are two main clusters of C. trachomatis (A and B) the cluster A divided into two subclusters, while cluster B contain two subclusters. Conclusions: Genotyping of C. trachomatis is important to study the pathogenesis and epidemiology of genital chlamydia infection.