The IBEX knowledge-base a community resource enabling adoption and development of immunofluorescence imaging methods
The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D and 3D immunofluorescence imaging methods. The design of the Knowledge-Base is modeled after efforts in the open-source software community and includes three facets: a development platform (GitHub), static website, and service for data archiving. The Knowledge-Base facilitates the practice of open science throughout the research life cycle by providing validation data for recommended and non-recommended reagents, such as primary and secondary antibodies. In addition to reporting negative data, the Knowledge-Base empowers method adoption and evolution by providing a venue for sharing protocols, videos, datasets, software, and publications. A dedicated discussion forum fosters a sense of community among researchers while addressing questions not covered in published manuscripts. Together, scientists from around the world are advancing scientific discovery at a faster pace, reducing wasted time and effort, and instilling greater confidence in the resulting data.
Authors
- Ian T. Lamborn (ORCID: https://orcid.org/0009-0006-1490-3667)
- Spencer Fullam (ORCID: https://orcid.org/0000-0001-9012-3802)
- Hiroshi Ichise (ORCID: https://orcid.org/0000-0002-5187-810X)
- Kartika Padhan (ORCID: https://orcid.org/0000-0001-9561-4256)
- Kenneth J. Gollob (ORCID: https://orcid.org/0000-0003-4184-3867)
- Edward C. Schrom (ORCID: https://orcid.org/0000-0002-1793-6433)
- Joshua Croteau (ORCID: https://orcid.org/0000-0002-8142-0482)
- Ryan V. Moriarty (ORCID: https://orcid.org/0000-0001-7100-1906)
- Yuri Lin (ORCID: https://orcid.org/0009-0006-9784-2694)
- Kirsten Remmert (ORCID: https://orcid.org/0009-0004-0668-4164)
- Jonathan M. Hernandez (ORCID: https://orcid.org/0000-0001-7529-0557)
- Leanne Arakkal (ORCID: https://orcid.org/0000-0001-9189-7172)
- Andrea J. Radtke (ORCID: https://orcid.org/0000-0003-4379-8967)
- Brianna T. Wachter (ORCID: https://orcid.org/0000-0002-2950-2683)
- Alexandra Y. Kreins (ORCID: https://orcid.org/0000-0001-8748-5837)
- Jyh Liang Hor (ORCID: https://orcid.org/0000-0001-6721-4204)
- Ryan B. MacDonald (ORCID: https://orcid.org/0000-0003-4194-8925)
- Armando Arroyo-Mejías (ORCID: https://orcid.org/0000-0002-0835-911X)
- Zhixin Jing (ORCID: https://orcid.org/0000-0003-1118-7432)
- Kátia L.P. Morais (ORCID: https://orcid.org/0000-0001-8877-5887)
- Beatrice Clark (ORCID: https://orcid.org/0009-0003-9817-7874)
- Rebecca T. Beuschel (ORCID: https://orcid.org/0000-0002-3882-457X)
- Ifeanyichukwu U. Anidi (ORCID: https://orcid.org/0000-0002-2692-1225)
- Colin J. Chu (ORCID: https://orcid.org/0000-0003-2088-8310)
- Michael Y. Gerner (ORCID: https://orcid.org/0000-0001-5406-8308)
- Fabian Coscia (ORCID: https://orcid.org/0000-0002-2244-5081)
- Aleksandra Lunich
- Chen Makranz
- Salome Stierli
- Saven Denha
- Juan M Ocampo-Godinez
- Luz Jg
- Jake Colautti
- Rose Dever
- Emily Speranza
- Ziv Yaniv
- Vitoria Murakami Olyntho
- Joshua FE Koenig
- Tibor Z Verse
- Anita Gola
- Megan Vierhout
- Nathan Richoz
- Lihong Shi
- Adam K Wade-Vallance
- Rochelle M Shih
- Menna R Clatworthy
- Wanjing Shang
- Sarah A Teichmann
- Nathan Zangger
- Walderez O Dutra
- Katy Börner
- Vivien I Maltez
- Aanandita Kothurkar
- Ronald N Germain
- Rosa K Kortekaas
- John E McDonough
- Evelyn Kandov
- Annette Oxenius
- Wolfgang Kastenmüller
- Sonja Fritzsche
- Danny Jonigk
Institutions
- Universidade Federal de Minas Gerais (BR)
- Rush University Medical Center (US)
- National Institutes of Health (US)
- Cleveland Clinic (US)
- MRC Laboratory of Molecular Biology (GB)
- Morpho (United States) (US)
- Universidade de São Paulo (BR)
- University of Washington (US)
- University of Zurich (CH)
- University of Cambridge (GB)
- University of Würzburg (DE)
- Hospital Israelita Albert Einstein (BR)
- Cancer Institute (WIA) (IN)
- ETH Zurich (CH)
- University of California San Diego (US)
- Humboldt-Universität zu Berlin (DE)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (US)
- NIHR Moorfields Biomedical Research Centre (GB)
- BioLegend (United States) (US)
- Intelligent Systems Research (United States) (US)
- National Heart, Lung, and Blood Institute (US)
- Cleveland Clinic Florida (US)
- Czech Academy of Sciences, Institute of Microbiology (CZ)
- Vistec Electron Beam (Germany) (DE)
- German Center for Lung Research (DE)
- National Institute of Allergy and Infectious Diseases (US)
- Stem Cell Institute (PA)
- Immune Regulation (United Kingdom) (GB)
- Institute for Respiratory Health (AU)
- Advanced Tissue (United States) (US)
- University College London (GB)
- Indiana University (US)
- Rockefeller University (US)
- RWTH Aachen University (DE)
- Universidad Nacional Autónoma de México (MX)
- McMaster University (CA)
Publication Details
- Journal
- eLife
- Published
- 2026-01-07
- DOI
- https://doi.org/10.7554/elife.105737.3
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00