Itracanazole-loaded Spanlastics: Preparation, In-vitro Characterization and Antifungal Activity Study

Introduction: This study investigates the process of loading itraconazole (ITCZ) into span-lastic nanovesicles, which may increase its permeability and bioavailability. The objective of this study was the formulation and characterization of itraconazole-loaded spanlastics (ITCZ -SPs). Methods: Itraconazole-loaded spanlastics (ITCZ -SPs) were formulated using the ethanol injection method. The composition of the formulation included Tween 60 as an edge activator in various ratios and Span 60 as a nonionic surfactant. These formulations employed Soluplus as a stabilizer. Particle size (PS), polydispersity index (PDI), and entrapment efficiency (%EE) were all considered in the evaluation process, and then zeta potential was applied to the formulation. Additionally, this formula-tion was compared with capsules from three different companies that are currently available on the market. Results: The selected formulation showed a particle size of about 99 nm, an EE of about 81%, a PDI of about 0.263, and a zeta potential of -26.07 ± 0.89 mV. Among the tested samples, only the optimal formulation exhibited a distinct response against C. tropicalis at a 100% concentration, compared to the samples of the marketed drug. Conclusion: When combined, ITCZ-SPs significantly enhanced the solubility and permeability of ITCZ, offering a promising nanoplatform for hydrophobic drug delivery.