Seasonal Shifts in Water Utilization Strategies of Typical Desert Plants in a Desert Oasis Revealed by Hydrogen and Oxygen Stable Isotopes and Leaf δ13C

Understanding seasonal water acquisition strategies of desert plants is critical for predicting vegetation resilience under increasing hydrological stress in arid inland river basins. In hyper-arid oases, strong evaporative demand and declining groundwater levels impose tightly coupled constraints on plant water uptake across soil-plant-atmosphere continua. In this study, we combined hydrogen and oxygen stable isotopes, Bayesian mixing models, soil moisture measurements and groundwater monitoring, and leaf δ¹³C analysis to quantify monthly water-source contributions and long-term water-use efficiency of three dominant species (<i>Reaumuria soongarica</i>, <i>Tamarix ramosissima</i>, and <i>Populus euphratica</i>) in the Ejina Oasis. Clear ecohydrological niche differentiation was evident among the three species. <i>R. soongarica</i> exhibited moderate temporal flexibility by integrating shallow and deep soil water with episodic groundwater use, whereas <i>T. ramosissima</i> adopted a vertically integrated and hydraulically plastic strategy combining precipitation, multi-depth soil water, and groundwater. In contrast, <i>P. euphratica</i> followed a conservative strategy, relying predominantly on deep soil water with only minor and transient inputs from precipitation and groundwater. Across species and seasons, deep vadose-zone soil water (120-200 cm) consistently acted as the most stable and influential reservoir, buffering seasonal drought and sustaining transpiration. <i>T. ramosissima</i> maintained the highest intrinsic water-use efficiency, and <i>P. euphratica</i> exhibited consistently lower efficiency associated with sustained access to stable deep soil water. These contrasting strategies reveal multiple pathways of hydraulic stability and plasticity that underpin vegetation persistence under progressive groundwater depletion. By linking water-source partitioning with physiological regulation, this study provides a mechanistic basis for understanding plant water-use strategies and informs ecological water management and species-specific restoration in hyper-arid inland oases.