Metagenomic Surveillance of Blood-Fed Mosquitoes for Assessing Zoonotic Risk in Managed Animal Settings

This pilot study demonstrates that blood-fed mosquitoes can serve as effective non-invasive biological samplers for detecting environmental DNA signatures of potential zoonotic pathogens in managed animal settings. While the detection of pathogen-associated genomic fragments does not confirm active infection or transmission, the findings highlight the utility of mosquito-based metagenomic surveillance as an early warning and risk-detection tool within a One Health framework. Integrating such approaches with targeted diagnostics and epidemiological surveillance may strengthen preparedness for emerging vector-borne zoonotic threats.