The enzymes DisoNNex and TrypLE are interchangeable as harvesting agents for adipose tissue−derived mesenchymal stromal cells
DisoNNex is a relevant alternative to TrypLE for ASC harvest, resulting in equivalent yields and cell quality under the tested conditions. These findings support the potential of DisoNNex in GMP-compliant manufacturing of ASC-based cell therapies and provide starting conditions for its implementation in ASC processes. Nonetheless, full comparability studies within an established production process are pending.
Authors
- Lisbeth Drozd Højgaard (ORCID: https://orcid.org/0000-0002-1052-3337)
- Katrine Bugge (ORCID: https://orcid.org/0000-0002-6286-6243)
- Ellen Mønsted Johansen (ORCID: https://orcid.org/0000-0001-8300-4100)
- Casper Wilkens (ORCID: https://orcid.org/0000-0001-7692-067X)
- Stine Bangsgaard
- Laura Lykke Lethager
- Morten Juhl Nørgaard
- Tu Hu
Institutions
- Novo Nordisk (Denmark) (DK)
- Copenhagen University Hospital (DK)
- Quality of Life Research Center (DK)
Publication Details
- Journal
- Cytotherapy
- Published
- 2026-01-23
- DOI
- https://doi.org/10.1016/j.jcyt.2026.102066
- Primary Topic
- Mesenchymal stem cell research
- Type
- article
- Field-Weighted Citation Impact
- 0.00