Cognatiyoonia denitrificans sp. nov., isolated from seawater

A novel Gram-stain-negative, rod-shaped, non-spore-forming, non-flagellated, strictly aerobic strain, designated as R2331^T, was isolated from coastal seawater of Yantai, P.R. China (127.59° E, 37.46° N). The organism grew optimally at 28-32 °C, at pH 7.0 and in the presence of 3.0% (w/v) NaCl. Strain R2331^T contained ubiquinone-10 as the predominant respiratory quinone and C_{18 : 1} <i> ω</i>7<i>c</i> and/or C_{18 : 1} <i> ω</i>6<i>c</i> as the major fatty acids. The predominant polar lipids of strain R2331^T were phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol and two unidentified aminolipids. The DNA G+C content of strain R2331^T was 59.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to <i>Yoonia ponticola</i> KCTC 42133^T with 96.3% sequence similarity. Calculated ANI values, dDDH values and AAI values between strain R2331^T and the phylogenetically related species were in the range 71.4-73.7 %, 18.0-19.4 % and 67.2-71.1 %, respectively. Strain R2331^T fell within the cluster of the genus <i>Cognatiyoonia</i> in the phylogenomic tree. Based on polyphasic analyses, strain R2331^T represents a novel species of the genus <i>Cognatiyoonia</i>, for which the name <i>Cognatiyoonia denitrificans</i> sp. nov. is proposed. The type strain is R2331^T (=KCTC 8319^T=MCCC 1K04209^T).