Herbiconiux salviae sp. nov., isolated from the flower of Salvia splendens

An aerobic, Gram-stain-positive and non-motile actinobacterial strain, designated SALV-R1^T, was isolated from flower of <i>Salvia splendens</i> in Muan, Republic of Korea. Growth was observed at 10-30 °C (optimum, 28 °C) and pH 7.0-9.0 (optimum, pH 8.0) with up to 3% (w/v) NaCl (optimum, 1%). blast analysis of 16S rRNA gene sequences revealed the highest sequence similarity of strain SALV-R1^T to <i>Herbiconiux oxytropis</i> CPCC 203406^T (98.76%) followed by <i>Herbiconiux flava</i> VKM Ac-2058 (98.69%) and '<i>Herbiconiux gentiana</i>' CPCC 205716^T (98.69%). According to the 16S rRNA gene-based phylogenetic tree, strain SALV-R1^T formed a clade with <i>H. flava</i> NBRC 164003^T and '<i>H. gentiana</i>' CPCC 205716^T, which subsequently clustered with <i>H. oxytropis</i> CPCC 203406^T. Strain SALV-R1^T was closely related to <i>Herbiconiux moechotypicola</i> KCTC 19653^T as well as <i>H. flava</i> NBRC 164003^T and '<i>H. gentiana</i>' CPCC 205716^T based on phylogenomic tree. OrthoANI and digital DNA-DNA hybridization values of strain SALV-R1^T with the closely related strains were lower than 81.2 and 24.8%, respectively. The major respiratory menaquinones were MK-11 and MK-10. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and glycolipid. The major fatty acids (>10%) of strain SALV-R1^T were anteiso-C_15:0, iso-C_16:0 and anteiso-C_17:0. The cell wall peptidoglycan contained cross-linked l-diaminobutyric acid (type B2<i>γ</i>). The phenotypic, chemotaxonomic and genotypic data obtained in this study showed that strain SALV-R1^T represents a novel species of the genus <i>Herbiconiux</i>, for which the name <i>Herbiconiux salviae</i> sp. nov. (type strain SALV-R1^T=KACC 21604^T=NBRC 116987^T) is proposed.