The impact of STING signaling on myeloid cells highlights macrophages as the major player in controlling Brucella abortus
Infected LysM-SKO mice, but not SKO-CD11c, showed an increase in CFU compared to infected WT animals. <i>In vitro</i>, the supernatant of infected BMDMs and BMDCs from LysM-SKO and CD11c-SKO animals showed decreased LDH and IL1-β and TNF-α when compared with WT cells. The absence of STING in infected BMDMs decreased the amount of cleaved caspase-1, -11 and GSDMD, evaluated by western blot analysis. The effect of lack of STING was more profound in macrophages than dendritic cells on innate signaling. In both BMDC and BMDM, the absence of STING did not affect NLRP3 expression. Together, these data indicate that STING signaling in macrophages and not in dendritic cells is important to control <i>Brucella</i> infection <i>in vivo</i> and impacts on inflammasome signaling pathway.
Authors
- Gustavo P. Amarante‐Mendes (ORCID: https://orcid.org/0000-0002-7851-6205)
- Fábio Mambelli (ORCID: https://orcid.org/0000-0002-8956-7219)
- Glen N. Barber (ORCID: https://orcid.org/0000-0002-4703-0931)
- Sérgio C. Oliveira (ORCID: https://orcid.org/0000-0003-4062-5577)
- Marcelo Pires Amaral (ORCID: https://orcid.org/0000-0002-5335-6196)
- Kívia Queiroz de Andrade (ORCID: https://orcid.org/0000-0003-2034-1956)
- Fábio Marinho
- Nina M. G. P. de Queiroz
Institutions
- Universidade Federal de Minas Gerais (BR)
- Instituto Federal de Educação, Ciência e Tecnologia de Minas Gerais (BR)
- Institut Pasteur (FR)
- Universidade de São Paulo (BR)
- Immunité et Cancer (FR)
- Institute of Biomedical Science (GB)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2026-01-21
- DOI
- https://doi.org/10.3389/fimmu.2025.1727400
- Primary Topic
- Brucella: diagnosis, epidemiology, treatment
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- National Institutes of Health