<sup>1</sup>H-NMR-Based Metabolomic Profiling and Phylogenetic Analysis of <i>Dendrobium</i> Species Identify Lineage-Correlated Metabolites in the Main Clades

Dendrobium Sw. is one of the largest genera in the Orchidaceae. Most species belong to one of two major clades (the Asian and the Australasian clades) based on morphology and phylogenetic analyses using DNA sequences. Several Dendrobium species in the Asian clade are used in traditional herbal medicine, and many compounds have been isolated from them (e.g., phenanthrene derivatives, bibenzyl derivatives, and polysaccharides). Conversely, there are only a few reports on the compounds contained in the Australasian clade species. Due to its size and diversity, the Australasian clade could be expected to contain compounds of potential medicinal value as well. Previously, we constructed the HPLC profile of 18 Dendrobium species and identified the phenanthrene derivative 1,5-dimethoxyphenanthrene-2,7-diol (1) as a characteristic compound in certain species of the Australasian clade. In this study, we performed metabolic analyses based on ¹H-NMR to identify lineage-correlated metabolites for the Australasian clade. NMR profiling analysis also showed that 1 is a characteristic compound of the Australasian clade species. Additionally, pinoresinol (2) was predominantly detected in the Australasian clade. While syringaresinol (3) was widely detected in species from both clades, specimens from the Australasian clade tended to have higher concentrations. The simple ¹H-NMR profiling method enables rapid comparison of metabolites across multiple species, providing new insights into metabolic differences associated with evolutionary lineages that were not detectable by the previous HPLC profiling.