The Tonoplast Topology Index—a new metric for describing vacuole organization
We present the protocol for TTI determination as a two-stage semi-automated procedure involving microscopic image analysis employing an ImageJ macro and subsequent processing of numeric data in the Jupyter Notebook environment, together with benchmarking image data. Since this implementation is freeware-based, platform-independent and (relatively) user-friendly, we hope it will find its use as a high throughput, added value alternative to the VMI metric.
Authors
- George Alexandru Caldarescu (ORCID: https://orcid.org/0000-0002-2839-5302)
- Radek Bezvoda (ORCID: https://orcid.org/0000-0002-9540-7422)
- Fatima Cvrčková (ORCID: https://orcid.org/0000-0002-5560-8473)
- Helena Kočová
Institutions
- Charles University (CZ)
- Max Planck Institute for Plant Breeding Research (DE)
Publication Details
- Journal
- Plant Methods
- Published
- 2026-01-21
- DOI
- https://doi.org/10.1186/s13007-025-01493-3
- Primary Topic
- Cellular transport and secretion
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Universität Heidelberg
- Grantová Agentura České Republiky
- European Regional Development Fund
- National Institute for Basic Biology