Influence of Alpine Forest Types on Soil Microbial Diversity and Soil Quality

Alpine forests are key regulators of soil biogeochemical cycles, yet the extent to which forest type constrains soil microbial diversity and soil quality in high-elevation regions remains insufficiently resolved. Here, we assessed how contrasting alpine forest types influence the taxonomic composition and diversity of soil microbial communities, identified the dominant environmental drivers, and evaluated soil quality along the southern slope of the Qilian Mountains. Six forest types were examined, including four monospecific stands (<i>Picea crassifolia</i>, QQ; <i>Betula</i> spp., HS; <i>Juniperus przewalskii</i>, YB; and <i>Pinus tabuliformis</i>, YS) and two mixed formations (mixed conifer-broadleaf, ZKHJ; and mixed broadleaved, KKHJ). Bacterial and fungal communities were characterized using Illumina high-throughput sequencing, while structural equation modeling (SEM) was used to identify primary drivers of diversity and principal component analysis (PCA) was applied to construct the minimum data set (MDS) for soil quality evaluation. Mixed forests consistently exhibited higher bacterial and fungal alpha diversity than pure stands. Environmental gradients were the strongest positive drivers of microbial diversity, whereas soil chemical properties and vegetation-related biotic factors exerted partially negative effects. Soil quality index (SQI) values ranked as follows: KKHJ (0.55) > ZKHJ (0.49) > YB (0.48) > HS (0.46) > YS (0.44) > QQ (0.43). The mixed broadleaved forest reached Grade IV (upper-intermediate level) soil quality, whereas the other forest types were classified as Grade III (intermediate). Mixed forests showed stronger capacities for organic matter accumulation and nutrient retention. These findings indicate that promoting mixed forest stands is critical for improving soil structure, nutrient retention, and microbial diversity in this alpine region. Accordingly, forest management should prioritize the development of mixed forests to enhance overall soil quality.