Comprehensive assessment of activity, specificity, and safety of hypercompact TnpB systems for gene editing
By comprehensively analyzing genome editing outcomes, we characterize TnpB systems for genome editing and identify ISYmu1 TnpB as an optimal miniature RNA-guided genome editors with balanced performance, highlighting its potential for therapeutic applications.
Authors
- Jiaxu Hong
- Jiazhi Hu (ORCID: https://orcid.org/0000-0002-6345-0039)
- Guanghai Xiang (ORCID: https://orcid.org/0000-0002-3495-1862)
- Shaopeng Yuan (ORCID: https://orcid.org/0000-0001-9554-1325)
- Yongyuan Huo
- Haoyi Wang
- Jing Sun
- Xinyi Liu
- Yuhong Wang
- Duan Liu
- Xichen Wan
- Shiwei Cao
- Changchang Xin
Institutions
- Chinese Academy of Sciences (CN)
- Peking University (CN)
- Institute for Stem Cell Biology and Regenerative Medicine (IN)
- Eye & ENT Hospital of Fudan University (CN)
- Chengdu University (CN)
- National Science Centre (PL)
- Shanghai Cell Therapy Research Institute (CN)
- Center for Life Sciences (CN)
- University of Chinese Academy of Sciences (CN)
- Institute of Zoology (CN)
Publication Details
- Journal
- Genome biology
- Published
- 2026-01-21
- DOI
- https://doi.org/10.1186/s13059-026-03949-8
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00