Anti- <i>Sporothrix brasiliensis</i> potential and anti-biofilm activity of <i>Psidium guajava</i> L. hydroacetone extract

<i>Sporothrix brasiliensis</i> is the primary cause of sporotrichosis in Brazil. Reports of therapeutic failures with itraconazole treatment exist. We evaluated the antifungal activity of the hydroacetone extract of <i>Psidium guajava</i> in planktonic cells and biofilms of <i>S. brasiliensis</i>. The extract was obtained from the leaves, and high-performance liquid chromatography was performed. Broth microdilution tests were performed according to the CLSI M38. The strains were subjected to biofilm formation, and the antifungal activity of the extract against a strong producer strain was determined. The extract inhibited planktonic cells with minimum inhibitory concentrations (MICs) of ≤ 2 to 8 µg/mL. The metabolic activity of the strain with strong biofilm formation was inhibited with MICs of 16 and 32 µg/mL. The anti-<i>Sporothrix</i> activity of the hydroacetone extract of <i>P. guajava</i> serves as a basis for future studies for developing new drugs for the treatment of sporotrichosis.