Unfairly forgotten deconvolution methods of combinatorial libraries

Since the introduction of split and pool synthesis, making it possible to create even billion-member combinatorial libraries, two deconvolution possibilities have been extensively used: the one-bead one-compound (OBOC) and the DNA-encoded methods. The originally available back search screening and the possibilities offered by the later-developed omission peptide libraries and the positional scanning have long been forgotten. Here, I compared the advantages and disadvantages of the above-mentioned methods, emphasizing the advantage of screening free dissolved molecules in the back search method.