Beyond the clot: how the biomolecular landscape of platelet-rich fibrin directs fibroblast functions

Platelet-Rich Fibrin (PRF) is a second-generation autologous blood concentrate widely applied in regenerative medicine and dentistry for its wound-healing potential. Its clinical applications span dermatology, plastic surgery, periodontology, implantology, and oral maxillofacial surgery, with growing evidence supporting its effectiveness in tissue regeneration. Fibroblasts, as central regulators of extracellular matrix synthesis and remodeling, angiogenesis, and inflammation, are important targets of PRF's regenerative effects. This review summarizes the recent evidence of role of PRF in regulation of fibroblast functions important for wound healing and inflammation. It highlights PRF as a biologically active scaffold that accelerates soft tissue repair, primarily through modulation of fibroblasts, positioning it as a promising adjunct in regenerative therapies.