Lab-on-a-Chip Metabolic Analysis Using Benchtop NMR Technology

Organ-on-chip (OoC) systems are advancing rapidly as physiologically relevant in vitro models. However, real-time, noninvasive metabolic monitoring tools remain lacking. While NMR offers a powerful solution, current setups are not compatible with the planar format of microfluidic chips. Here we introduce the first benchtop NMR spectrometer for real-time metabolic monitoring of cell cultures on microfluidic platforms, utilizing hyperpolarization via dissolution dynamic nuclear polarization. This work details modifications made to a commercial benchtop NMR spectrometer, including the design and fabrication of a microfluidic platform that enables precise injection of hyperpolarized substrates and continuous renewal of cell culture media. The platform integrates a radiofrequency coil for signal transmission and reception and incorporates a custom-built sample carrier. Preliminary NMR data acquired with this system demonstrate its feasibility for dynamic metabolic studies.