In vitro shoot induction of BesNO H 382 tobacco using benzyl amino purine

Besuki Na Oogst (BesNO) H 382 tobacco is a superior commodity intended for export as a raw material for cigar production in the international market from Jember Indonesia. The development of its seed development through tissue culture has advantages such as the ability to produce healthy and uniform seeds, pathogen-free and regardless of the season. This study aimed to determine the optimal concentration of benzyl amino purine (BAP) for in vitro shoot induction. The research using a Completely Randomized Design with four levels of BAP concentration ranging from 0 to 3 ppm. The findings showed that BAP concentrations between 1 and 3 ppm significantly influenced shoot induction of BesNO H 382 tobacco. Among these treatments, 3 ppm BAP was identified as the most effective concentration, with shoot emergence observed at 8.40 days, an explant weight of 4.44 g, and an average of 30 shoots per explant at 56 days after inoculation. These results provide valuable insights into the optimization of plant growth regulators from the Cytokinin group for optimal shoot induction of BesNO H382 tobacco which is still rarely reported with a new modification of the explant sterilization technique.