Semen Quality of Holstein Bulls and Expression of the AQP7, STK35, ITF27, and PRM1 Genes: Correlation Analysis

Evaluation of sperm-specific gene expression is a potential resource for diagnostics of impaired spermiogenesis, cryoresistance, and the fertility of these germ cells. These are important indicators of sperm quality, which should be taken into account when selecting breeding bulls for collecting semen intended for cryopreservation. Here, a correlation analysis of the expression levels of the AQP7, STK35, IFT27, and PRM1 genes with sperm quality indicators in native and decryopreserved sperm of Holstein bulls was carried out. We found a highly reliable positive correlation between the expression of AQP7, ITF27, and PRM1 with a number of spermatozoa viability indicators and a negative one with indicators characterizing the loss of their functionality. The results obtained can be used for developing transcriptional biomarkers of sperm quality in breeding bulls based on the parameters of cryotolerance and the fertilizing potential of spermatozoa.