Peculiarities of Cryopreservation and Decryopreservation of Primordial Germ Cells in the Pushkin Breed of Chickens

Optimization of the protocols for cryopreservation and subsequent thawing of primary germ cells in Pushkin breed chicken was carried out. It was detected that with a controlled cooling rate of 1°C/min and combination of penetrating and non-penetrating cryoprotectants, a maximal viability of the cells was 62.9% after their slow thawing at 37°C, which is significantly higher than the values observed during thawing at 4°C. The results obtained emphasize a critical role of thawing temperature in the cell survival, demonstrating that rapid thawing increases their viability by minimizing sublethal damages. The study suggests a more efficient approach to the preservation of poultry genetic resources and creates a potential for biotechnology in the field of animal husbandry.