Development, validation, and application of SYBR green-based qPCR assays for detection and quantification of tetA, tetB, and tetO genes in poultry and associated environments

These SYBR Green-based qPCR assays provide a robust, quantitative, and affordable tool for monitoring <i>tet</i> genes dissemination in poultry and associated environments. Their simplicity and reproducibility make them particularly suitable for large-scale surveillance programs and for use in settings where resources or access to probe-based platforms are limited.