A Collection of Recombinant Beta-Lactamases for Antibacterial Drug Screening

Genetic engineering approach has been used to create a collection of recombinant E. coli strains-producers of bacterial enzymes beta-lactamases related both to the existing natural analogues and to the mutant forms of natural enzymes. Currently, the collection includes beta-lactamases of the TEM (molecular class A), CTX-M (molecular class A), VIM (molecular class B), and NDM (molecular class B) types. A set of recombinant beta-lactamases can be used for screening new antibacterial drugs and inhibitors in vitro. The collection of beta-lactamase producers is included in the National Depository Bank of Live Systems “Noah’s Ark” of the Lomonosov Moscow State University.