Lanostane triterpenoids from the fruiting bodies of <i>Ganoderma multipileum</i> and their anti-inflammatory activities

A previously unreported lanostane-type triterpenoid designated as (25<i>S</i>)-3<i>β</i>,15<i>β</i>-dihydroxy-7,11,23-trioxo-lanost-8,17(20)-dien-26-oic acid (<b>1</b>) and fifteen known compounds (<b>2</b>-<b>16</b>), was obtained from <i>Ganoderma multipileum</i> fruiting bodies. Combined analysis of NMR and HRESIMS data, together with theoretical computations involving NMR chemical shift prediction and DP4+ analysis, the structural elucidation of the new compound was accomplished. Bioactivity screen was performed on all compounds to assess their anti-inflammatory activity. Notably, in LPS-stimulated RAW264.7 cells, compounds <b>4</b> and <b>5</b> inhibited the NO generation, yielding IC₅₀ values of 12.89 ± 0.50 and 27.55 ± 2.95 μM, respectively, with potency close to that of quercetin (IC₅₀: 11.80 ± 1.75 μM).