Engineering human bone marrow-derived mesenchymal stromal cell aggregates for enhanced extracellular vesicle secretion in a vertical-wheel bioreactor
VWBR-driven hydrodynamics promotes EV biogenesis from 3D hMSC aggregates, improving metabolic activity, EV cargo relevance, and functional efficacy. The resulting EVs exhibit therapeutic cargo capable of modulating neural inflammation. These findings advance understanding of dynamic aggregation on metabolic cues and EV production, demonstrating a scalable strategy for generating therapeutically potent hMSC-EVs for neuropathic and regenerative applications.
Authors
- D. Berry (ORCID: https://orcid.org/0000-0003-2672-0826)
- Breana Boirie
- M. P. Singh
- Li Sun
- Changchun Zeng
- Sunghoon Jung
- Yan Li
Institutions
- Florida State University (US)
- Florida A&M University - Florida State University College of Engineering (US)
- American Association of Colleges of Pharmacy (US)
- 490 BioTech (United States) (US)
- Florida Agricultural and Mechanical University (US)
Publication Details
- Journal
- Frontiers in Bioengineering and Biotechnology
- Published
- 2026-01-16
- DOI
- https://doi.org/10.3389/fbioe.2025.1664302
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- Florida State University
- Florida Agricultural and Mechanical University
- National Institutes of Health
- Directorate for Biological Sciences
- Division of Chemical, Bioengineering, Environmental, and Transport Systems