Single-cell proteomics of Arabidopsis leaf mesophyll reveals dynamic protein responses to water-deficit stress

The label-free scProteomic approach presented here represents a significant advance through the demonstration of a facile protoplast isolation method combined with deep and precise proteomic coverage of Arabidopsis leaf mesophyll cell types. We believe this study will serve as an informative reference to future plant scProteomic investigations.

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Publication Details

Journal
Genome biology
Published
2026-01-16
DOI
https://doi.org/10.1186/s13059-025-03919-6
Primary Topic
Advanced Proteomics Techniques and Applications
Type
article
Field-Weighted Citation Impact
0.00
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article

Single-cell proteomics of Arabidopsis leaf mesophyll reveals dynamic protein responses to water-deficit stress

Pranav Dawar, Kyle D. Nadeau, S. Williams, James M. Fulcher et al.
Genome biology
Advanced Proteomics Techniques and Applications
article

Single-cell proteomics of Arabidopsis leaf mesophyll reveals dynamic protein responses to water-deficit stress

Pranav Dawar, Kyle D. Nadeau, S. Williams, James M. Fulcher, Ljiljana Paša-Tolić, Amir H. Ahkami, Vimal Kumar Balasubramanian, Ying Zhu, Lye Meng Markillie
article en

Abstract

The label-free scProteomic approach presented here represents a significant advance through the demonstration of a facile protoplast isolation method combined with deep and precise proteomic coverage of Arabidopsis leaf mesophyll cell types. We believe this study will serve as an informative reference to future plant scProteomic investigations.

Genome biologyVol. 27(1)
Pacific Northwest National Laboratory (US), Environmental Molecular Sciences Laboratory (US), Genetic Technologies (Australia) (AU)
Clean water and sanitation
Openalex Percentile: Top 87%
Advanced Proteomics Techniques and Applications
DOIOpen Access PDFOpenAlex
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