HPLC Method Validation for Analysis of Sappanone A from Caesalpinia sappan L. Heartwood

In this experiment, we developed and validated the HPLC method for quantifying sappanone A from the heartwood of Caesalpinia sappan L. (Leguminosae). The extraction procedure based on sonication was established and the analysis method was optimized using a C18 column. Method validation was conducted in accordance with the KFDA validation guideline, evaluating linearity, accuracy, precision, repeatability, and limit of quantitation. All tested analytical parameters were within acceptable range, indicated that the developed HPLC method is reliable for the quantitative analysis of sappanone A of C. sappan heartwood.