Measuring Propidium Iodide Brightness as a Function of Viability: A High‐Throughput Quantitative Assessment of Hepatic Spheroids
A fixed-setting AO/PI imaging workflow yields a rapid, accessible proxy for hepatocyte spheroid viability that correlates with a functional readout. This approach is well-suited to high-throughput screening and method optimization.
Authors
- Clemens Mg
- A. W. Chen
- Joseph Sushil Rao (ORCID: https://orcid.org/0000-0002-0197-6107)
- Charles Y. Lee
- E. B. Finger
- Diane Tobolt
- JC Bischof
- Michael G. Megaly (ORCID: https://orcid.org/0009-0003-1198-6610)
- Srivasupradha Ramesh
- Benjamin Fisher
- Bat‐Erdene Namsrai
- Anudari Chimedtseren
Institutions
- University of North Carolina at Charlotte (US)
- University of Minnesota (US)
- University of Minnesota System (US)
Publication Details
- Journal
- Artificial Organs
- Published
- 2026-01-14
- DOI
- https://doi.org/10.1111/aor.70089
- Primary Topic
- Liver physiology and pathology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- National Institutes of Health