Validated UV-Visible Spectrophotometric Method for Quantitative Analysis of Glycyrrhizin

Aim: The study aimed to develop and validate a simple, accurate, precise and cost-effective UV-Visible spectrophotometric method for the estimation of Glycyrrhizin in bulk as well as in formulation. Methods: A UV-Visible spectrophotometric method for Glycyrrhizin was developed using HPLC grade water. The solution was scanned across the UV-Visible range of 200-800 nm to identify its wavelength of maximum absorbance. The linearity of the method was established using six calibration standards over a concentration range of 5–75 μg/mL. Three different quality control standard solutions of the Glycyrrhizin were used for establishing the accuracy and precision of the proposed method. Proposed method was validated using ICH Q2 (R1) guidelines on the basis of accuracy, precision, robustness, limit of Detection (LOD), and limit of Quantitation (LOQ). Results: Glycyrrhizin when dissolved in methanol and diluted by using HPLC grade water showed maximum absorbance at wavelength of 254 nm. The developed UV–Visible spectrophotometric method demonstrated excellent linearity across the concentration range of 5–75 μg/mL, with a Correlation coefficient (r²) of 0.999. The intra-day accuracy of the proposed UV–Visible Spectrophotometric method in terms of % Difference was in the range of +0.5487 to +1.8644 whereas the inter-day accuracy was in the range of +0.6250 to + 1.9096. The intra-day precision of the proposed UV–Visible spectrophotometric method in terms of % RSD was found to be in Between 0.2681 to 1.3558 whereas the inter-day precision values were in between 0.2581 to 1.3052. The variation (% RSD) during the robustness study of the proposed UV–Visible spectrophotometric method was found be below 2%. The limit of detection (LOD) and limit of quantitation (LOQ) of the proposed UV–Visible spectrophotometric method was found to be 1.7285 μg/mL and 5.2380 μg/mL respectively, ensuring adequate sensitivity for routine analysis. Proposed method was successfully used for the estimation of Glycyrrhizin in the formulation. Conclusion: The proposed UV-Visible spectrophotometric method for estimation of Glycyrrhizin is simple, sensitive, accurate, and cost effective. It is suitable for routine analysis of Glycyrrhizin in commercial formulation. Keywords: Glycyrrhizin, UV-Visible method, ICH Q2 (R1)