Simultaneous estimation of Linalool and Eugenol in herbal formulations using a validated RP-HPLC method: A green analytical approach

Linalool and Eugenol commonly found in herbal products happen to be major bioactive components because of their anti-inflammatory, antioxidant, as well as antimicrobial activities. For the quality control and standardization of these products, a strong analytical technique that truly is effective enough is necessary. The current study develops and validates a reversed-phase high-performance liquid chromatography method that is sufficiently simple, accurate, and precise. The method quantifies combined amounts of Linalool and Eugenol in the herbal formulations. A C18 column that flowed at 1.0 mL/min separated chromatographically using acetonitrile and water mobile phase (55:45, %v/v). The approach was validated in accordance with ICH Q2(R2) guidelines for parameters like linearity, specificity, LOQ, LOD, robustness, accuracy, and precision. For both of the analytes with correlation coefficients greater than 0.9957, the technique demonstrated quite good linearity. This linearity was present between about 2–30 µg/mL. AGREE, GAPI, NEMI, tools were used to evaluate the method's greenness. Concepts of White Analytical Chemistry (WAC) were brought in using RGB fast, as well as BAGI models. Sustainability, safety, and environmental footprint, for each of the method were assessed. The method developed was successfully used in commercial herbal products, proving its applicability for routine quality control purposes. • Eco-friendly RP-HPLC method for simultaneous assay of linalool and eugenol. • Method validated per ICH Q2(R2) with excellent accuracy, precision, and linearity. • Successfully applied for quality control of commercial herbal oil formulations. • Greenness assessed using GAPI, AGREE, NEMI, AMGS, RGB Fast, and BAGI tools. • Aligns with White Analytical Chemistry principles for sustainability in analysis.