EVALUATION OF THE EFFECTS OF SULFAMETHOXAZOLE ON ENDOTHELIAL AND FIBROBLAST CELLS

Objective: Sulfamethoxazole (SMX) is a sulfonamide derivative drug used in the treatment of bacterial infections. The potential role of SMX not only in the treatment of infections but also in tissue regeneration and wound healing has aroused interest in its potential therapeutic applications. In this study, we aimed to determine the effects of SMX on fibroblast and endothelial cell viability, migration and collagen gene expression. Material and Method: In this study, HUVEC was used as endothelial cell line and L929 as fibroblast cell line. Cell viability was evaluated by MTT assay, migration inhibition by wound healing assay and COL1A1 gene expression by qRT-PCR method. Result and Discussion: SMX was found to show some positive effects on endothelial and fibroblast cells to promote wound healing, but some concentrations may negatively affect cellular processes. More detailed in vitro research is needed to expand the therapeutic areas in which SMX is used.