Effects of low-serum adaptation on growth and protein expression in stably lactoferrin-overexpressing mammary alveolar cells
Across all the low-serum conditions, cells retained typical epithelial morphology (cytokeratin 18-positive) while exhibiting consistent <i>LTF</i> mRNA transcription levels, protein expression, and secretory output. These findings validate the scalable low-serum culturing of MAC-T cells and <i>in vitro</i> LTF synthesis.
Authors
- Yajing Ji (ORCID: https://orcid.org/0009-0004-1083-3935)
- Xiaoqian Cai (ORCID: https://orcid.org/0000-0003-3967-0741)
- G. Li
- Lijun Chen
- Zhenzhen Zhang (ORCID: https://orcid.org/0000-0002-9885-7453)
- Hui Wang (ORCID: https://orcid.org/0000-0002-5538-1838)
- Qiang Zhou (ORCID: https://orcid.org/0000-0002-5744-2761)
- Chunling Bai
- Ming Chen
- Junying Zhao
- Weicang Qiao
- Lei Yang
- Qiuying Wang
- Guanghua Su
Institutions
- Inner Mongolia University (CN)
- Beijing Academy of Food Sciences (CN)
- National Research Center for Maternal and Child Health (KZ)
- Dalian Polytechnic University (CN)
Publication Details
- Journal
- Frontiers in Bioengineering and Biotechnology
- Published
- 2026-01-15
- DOI
- https://doi.org/10.3389/fbioe.2026.1731548
- Primary Topic
- Infant Nutrition and Health
- Type
- article
- Field-Weighted Citation Impact
- 0.00